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Product Features:

• Ready-made lentiviral particles, just expose your target cells - EASY.

• Generate stable firefly luciferase Luc2 expressing cell lines FAST.

• Generate the BRIGHTEST cell lines 100 - >1000 ph/s/cell.

• Generate Luc2 gene expression in primary cells, non-dividing and dividing mammalian cells.

• Firefly luciferase Luc2 from pGL4 is the most sensitive reporter for deep tissue in vivo optical imaging.

• Best for in vivo imaging: Luc2 labeled tumor cells, stem cells, white blood cells.

Product Description:

Lenti Fire combines the most sensitive in vivo optical imaging reporter namely the firefly luciferase Luc2 construct  from the pGL4 plasmid (Promega) with the best gene delivery system, a third generation replication incompetent lentivirus.

Luc2 is the best and brightest firefly luciferase construct for deep tissue in vivo bioluminescence imaging. This enhanced luciferase is codon optimized for mammalian cell cytoplasmic expression. Luc2 emission spectrum peaks at 612nm in mammalian cells at 37C. The substrate is regular, water-soluble D-Luciferin. 

Luciola italica and other native luciferases are expressed in the peroxisome, and therefore poor photon emittors.   Gaussia and Renilla luciferase emit at 480nm. Any light <600nm is heavily absorbed by tissue.  GLuc and RLuc utilize Coelenterazine as a substrate which is not water-soluble, and produces short-lived, flash kinetics.

An Ubiquitin C promoter drives high constitutive expression of the transgene circumventing the issue of CMV promoter silencing, observed in certain cell lines.

Lentiviral vectors are safe, viral based gene delivery systems that generate long term, sustained expression of transgenes. Lentiviral vectors are able to stably integrate into quiescent, non-dividing as well as dividing cells without silencing and without immune response. Transgene efficiency of close to 100% is common and lentiviruses infect a broad array of species and the most resilient cells. 

Product Components:

YOU MAY ADD: 

- Custom Cell Line Transfection Service - $1975.00

- Polybrene - $79.95

- Puromycin - $59.95

Packaging:

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Limited Use License: 

Use of this product requires acceptance of the Promega Limited Use License which can be found here: Limited Use License

NOTE: This product is distributed for laboratory research only, and is strictly excluded from clinical use.

  Lenti-Fire product manual             Lentivirus White Paper