Cell transfection is a method that manipulates the gene expression
of cells. Transfected cells can be modulated to either express a
transgene/protein/reporter of interest or, suppress the expression of a
particular gene/protein via RNAi. Compared to transfection methods such as cationic lipids,
electroporation, or microinjection, viral expression systems are
the most efficient. Transduction is the process by which genetic material, e.g.DNA or siRNA, is inserted into a cell by a
virus. Lentiviral vectors are able to stably
integrate into quiescent, non-dividing as well as dividing cells without
silencing, and with low immunogenicity. Lentiviruses have a transgene
efficiency of >95% and have tropism for a broad array of mammalian species. A
Lentivirus distinguishes itself with long term, sustained expression of
transgenes. These features allow for fast and easy transduction of immortal and
primary cell lines.
- We custom design any
gene/protein/reporter/siRNA constructs.We also have bi-cistronic and tri-cistronic vectors. These custom constructs can be used for
plasmid transfections or lentiviral transductions.
- We custom produce lentivirus
for your gene and/or imaging reporter or siRNA of interest or; choose our ready-made luc2 or GFP lentivirus.
- We also offer custom lentiviral, stable transfection for
your cell line of interest.
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