Item Name: Luciferin Sodium 1g

Catalog ID: 30

Product Features:

D-luciferin: High quality grade,>99%HPLC, in vivo tested, manufactured in a GMP facility.  L-luciferin <0.5%, detected and quantified by chiral HPLC. Low dehydroluciferin levels.

Na⁺-salt solubility in water is 100mg/mL.

Recommended injection dose: 150 mg/kg IP (3mg for a 20g mouse).

Product Description:

D-luciferin is the substrate of Firefly luciferase. The luciferase enzyme catalyzes the oxidization of D-luciferin to oxyluciferin in the presence of ATP, Mg²⁺, and oxygen with production of a yellow-green light, shifting to red light in vivo at 37^oC. Since ATP is required as a co-factor, luciferase can be used as an indicator of the presence of energy or “life” and function as a life-death stain. D-luciferin is also the substrate for clickbeetle red and clickbeetle green luciferase.

Today, luciferin is a common reagent used in the Life Science “in vivo Imaging” arena. Luciferase labeled tumor cells, infectious disease, stem cells, metabolic disease or T-lymphocytes are inoculated in research animals (mouse and rat).  Upon injection of the substrate D-luciferin; disease and drug efficacy can be monitored non-invasively in these life animals with CCD based scanner technique called in vivo bioluminescence imaging.

Frequent in vitro uses of D-luciferin include ATP assays, luciferase assays, inducible gene reporter assays, high throughput sequencing, hygiene and environmental contamination assays.

Packaging:

Easy to reconstitute 50ml bottle under Argon gas.

Storage:

D-luciferin salt is best stored in an amber vial under argon or nitrogen gas at -20^oC for at least 1 year.

Service:

We accommodate, fast shipping, same batch and bulk quantity orders.

Certificate of Analysis

D-luciferin in vivo imaging methods - white paper